Journal: Proteomics

Article Title: Evaluation of microarray surfaces and arraying parameters for autoantibody profiling

doi: 10.1002/pmic.200800146

Figure Lengend Snippet: Slide surfaces evaluated for printing autoantigen microarrays

Article Snippet: Many slide surfaces did not have specific protocols recommended for printing and probing microarrays although some manufacturers, including Whatman, Sigma (for SigmaScreen™APS slide), and ArrayIt ® , recommended the use of proprietary print, block, and/or wash buffers.

Techniques: Modification, Binding Assay, In Situ Hybridization, Immunohistochemistry, In Vitro, Diagnostic Assay

Journal: Proteomics

Article Title: Evaluation of microarray surfaces and arraying parameters for autoantibody profiling

doi: 10.1002/pmic.200800146

Figure Lengend Snippet: Slide surfaces evaluated for printing autoantigen microarrays

Article Snippet: Slides were probed with a His-Tag mAb (Novagen) and Cy3-conjugated GAM IgG and IgM (Jackson ImmunoResearch Laboratories). table ft1 table-wrap mode="anchored" t5 caption a7 Slide Manufacturer Surface coating Intended use a) FAST Whatman NC-based polymer Protein microarrays b) PATH GenTel ® BioSciences Ultra-thin NC Protein microarrays Protein Glass NUNC Modified polymer Protein microarrays Low Background Aldehyde MicroSurfaces Aldehyde monolayer Protein, peptide, antibody arrays HydroGel Perkin Elmer Acrylamide-based polymer Protein microarrays a) Zeta-Grip™ Chips Miragene Hydrophobic polymer Protein microarrays a) CodeLink Activated Slides Amersham Hydrophilic, amine reactive polymer Amine binding a) SuperProtein ArrayIt Hydrophobic polymer Protein microarrays a) SuperEpoxy ArrayIt Epoxide groups Oligonucleotide, cDNA, protein, cell, tissue arrays b) SuperEpoxy2 ArrayIt Epoxide groups Oligonucleotide, cDNA, protein, cell, tissue arrays SuperAldehyde ArrayIt Primary aldehyde groups Oligonucleotide, cDNA, protein, cell, tissue arrays a) SuperNitro ArrayIt NC Protein microarrays a) Poly- l -lysine CEL Associates Poly- l -lysine DNA microarrays a) Acrylic CEL Associates Acrylic amine DNA microarrays Hydrazine CEL Associates Hydrazine DNA microarrays Mercapto CEL Associates Mercapto silane DNA microarrays Epoxy CEL Associates Epoxide groups DNA microarrays Amine CEL Associates 3-Aminopropyl groups DNA microarrays Carboxyl CEL Associates Carboxyl groups DNA microarrays Silylated (aldehyde) CEL Associates Reactive aldehyde groups In situ hybridization, immunohistochemistry, DNA a) Silanated (amine) CEL Associates Amine reactive groups In situ hybridization, immunohistochemistry Aldehyde CEL Associates Reactive Aldehyde groups Oligonucleotide microarrays a) Silane-Prep Sigma Aminoalkylsilane In vitro diagnostic use SigmaScreen APS Sigma Aminopropylsilane DNA microarrays Open in a separate window a) Slides passed the initial screen and evaluated for CV. b) Slides tested after initial screens had been performed.

Techniques: Modification, Binding Assay, In Situ Hybridization, Immunohistochemistry, In Vitro, Diagnostic Assay